Os benefícios da modalidade boccia em jovens portadores de deficiência

O presente estudo aborda os benefícios da modalidade Boccia em jovens portadores de deficiência, numa cooperativa, CERCIMIRA, localizada no concelho de Mira. Deste forma o objectivo principal passa por fomentar a participação e inserção de jovens, que comportem, vários tipos de deficiência mental de diferentes níveis (moderado, grave e muito grave), com expressões distintas nos quadros de vista cognitivo e/ou motor, numa modalidade adequada às suas capacidades físicas e cognitivas. Para que se alcancem os objectivos propostos, bem como se respondessem aos problemas deste estudo, utilizou-se como procedimentos metodológicos a pesquisa bibliográfica acerca de estudos idênticos, bem como a modalidade Boccia, conceitos fundamentais para a execução deste trabalho, a através da pesquisa em campo, pesquisa essa que se baseou em várias sessões de prática da modalidade com os utentes da CERCIMIRA, de forma a poder verificar a capacidade dos utentes de praticarem esta modalidade. Foram avaliados os resultados dos 15 participantes no nível de desempenho motor do lançamento de Boccia, antes e após das 18 sessões leccionadas na instituição (intervenção pedagógica com o grupo de portadores de deficiência). Os resultados obtidos mostram os efeitos positivos na melhoria do desempenho motor na tarefa de Boccia requerida, ou seja, a efectiva aprendizagem ao longo das sessões leccionadas. Adicionalmente, nos inquéritos realizados, constatamos o nível motivacional e interesse dos utentes na prática do Boccia na instituição são apresentados em 6gráficos, apontando que a prática desta modalidade por pessoas com deficiências múltiplas é possível e que existe um grau de melhoria desde a primeira sessão até à última

Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.

This Article contains an error in the description of the data presented in Figure 2. Each blot demonstrating a protein of interest, or of its phosphorylated form, is matched with the expression of β-actin, used as loading control. The majority of the proteins were separated in different gels, apart from proteins p105, p50 and Bcl-xL which were run in the same gel and have the same loading control. As a result, the Figure 2 legend, “Ionizing radiation induces macrophage NF-κB activation and increases Bcl-xL expression. (A) Evaluation of RelA phosphorylation (Ser536) and RelB, cRel, p100/p52 and p105/p50 subunit expression, 1 and 6 h after irradiation (2, 6 and 10 Gy). (B) RelB nuclear translocation 6 h after macrophage irradiation (10 Gy). Histone deacetylase 1 (HDAC1) and β-actin were used as loading controls for nuclear and cytoplasmic fractions, respectively. (C) Evaluation of Bcl2 and Bcl-xL expression after macrophage irradiation. Western blot images are representative of protein expression/phosphorylation status in distinct donors (at least n = 4), evaluated in two independent experiments.” should read: “Ionizing radiation induces macrophage NF-κB activation and increases Bcl-xL expression. (A) Evaluation of RelA phosphorylation (Ser536) and RelB, cRel, p100/p52 and p105/p50 subunit expression, 1 and 6 h after irradiation (2, 6 and 10 Gy). (B) RelB nuclear translocation 6 h after macrophage irradiation (10 Gy). Histone deacetylase 1 (HDAC1) and β-actin were used as loading controls for nuclear and cytoplasmic fractions, respectively. (C) Evaluation of Bcl2 and Bcl-xL expression after macrophage irradiation. Western blot images are representative of protein expression/phosphorylation status in distinct donors (at least n = 4), evaluated in two independent experiments. The β-actin loading control of the panels comprised by p105, p50 (2A) and Bcl-xL (2C) is the same, since proteins were separated in the same gel electrophoresis.

Macrophage Resistance to Ionizing Radiation Exposure Is Accompanied by Decreased Cathepsin D and Increased Transferrin Receptor 1 Expression.

PurposeTo identify a molecular signature of macrophages exposed to clinically relevant ionizing radiation (IR) doses, mirroring radiotherapy sessions.MethodsHuman monocyte-derived macrophages were exposed to 2 Gy/ fraction/ day for 5 days, mimicking one week of cancer patient's radiotherapy. Protein expression profile by proteomics was performed.ResultsA gene ontology analysis revealed that radiation-induced protein changes are associated with metabolic alterations, which were further supported by a reduction of both cellular ATP levels and glucose uptake. Most of the radiation-induced deregulated targets exhibited a decreased expression, as was the case of cathepsin D, a lysosomal protease associated with cell death, which was validated by Western blot. We also found that irradiated macrophages exhibited an increased expression of the transferrin receptor 1 (TfR1), which is responsible for the uptake of transferrin-bound iron. TfR1 upregulation was also found in tumor-associated mouse macrophages upon tumor irradiation. In vitro irradiated macrophages also presented a trend for increased divalent metal transporter 1 (DMT1), which transports iron from the endosome to the cytosol, and a significant increase in iron release.ConclusionsIrradiated macrophages present lower ATP levels and glucose uptake, and exhibit decreased cathepsin D expression, while increasing TfR1 expression and altering iron metabolism

CENTR(AR): pulmões em andamento: manual de atividade física domiciliário

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